crl 1435 Search Results


prostatic carcinoma cell lines  (ATCC)
99
ATCC prostatic carcinoma cell lines
Prostatic Carcinoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human prostate adenocarcinoma cell line  (ATCC)
97
ATCC human prostate adenocarcinoma cell line
Human Prostate Adenocarcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pc3 luciferase pc3 luc2  (ATCC)
92
ATCC pc3 luciferase pc3 luc2
Pc3 Luciferase Pc3 Luc2, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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distribution  (ATCC)
90
ATCC distribution
Distribution, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pc3  (ATCC)
94
ATCC pc3
Nanoparticle tracking analysis and EV morphology. NTA was performed on two biological replicates (i.e., Lot 1 and Lot 2) of ( a ) hTERT-immortalized MSC EVs and ( b ) <t>PC3</t> EVs to analyze relative concentration (particles/mL) and size distribution. Dashed lines indicate values of 50 nm and 200 nm. ( c ) The average % of EVs from each lot that measured between 50–200 nm in size and 200+ nm in size. n = 6 (2 biological replicates and each biological replicate was measured in triplicate). ** = p < 0.01. Representative TEM images show the appearance of ( d ) hTERT-immortalized MSC EVs and ( e ) <t>PC</t> <t>3</t> EVs. Scale bar = 100 nm.
Pc3, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pc-3 cell line crl-1435  (ScienCell)
90
ScienCell pc-3 cell line crl-1435
Nanoparticle tracking analysis and EV morphology. NTA was performed on two biological replicates (i.e., Lot 1 and Lot 2) of ( a ) hTERT-immortalized MSC EVs and ( b ) <t>PC3</t> EVs to analyze relative concentration (particles/mL) and size distribution. Dashed lines indicate values of 50 nm and 200 nm. ( c ) The average % of EVs from each lot that measured between 50–200 nm in size and 200+ nm in size. n = 6 (2 biological replicates and each biological replicate was measured in triplicate). ** = p < 0.01. Representative TEM images show the appearance of ( d ) hTERT-immortalized MSC EVs and ( e ) <t>PC</t> <t>3</t> EVs. Scale bar = 100 nm.
Pc 3 Cell Line Crl 1435, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Nanoparticle tracking analysis and EV morphology. NTA was performed on two biological replicates (i.e., Lot 1 and Lot 2) of ( a ) hTERT-immortalized MSC EVs and ( b ) PC3 EVs to analyze relative concentration (particles/mL) and size distribution. Dashed lines indicate values of 50 nm and 200 nm. ( c ) The average % of EVs from each lot that measured between 50–200 nm in size and 200+ nm in size. n = 6 (2 biological replicates and each biological replicate was measured in triplicate). ** = p < 0.01. Representative TEM images show the appearance of ( d ) hTERT-immortalized MSC EVs and ( e ) PC 3 EVs. Scale bar = 100 nm.

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: Nanoparticle tracking analysis and EV morphology. NTA was performed on two biological replicates (i.e., Lot 1 and Lot 2) of ( a ) hTERT-immortalized MSC EVs and ( b ) PC3 EVs to analyze relative concentration (particles/mL) and size distribution. Dashed lines indicate values of 50 nm and 200 nm. ( c ) The average % of EVs from each lot that measured between 50–200 nm in size and 200+ nm in size. n = 6 (2 biological replicates and each biological replicate was measured in triplicate). ** = p < 0.01. Representative TEM images show the appearance of ( d ) hTERT-immortalized MSC EVs and ( e ) PC 3 EVs. Scale bar = 100 nm.

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXMTM), PC3 (ATCC ® CRL-1435-EXMTM), and HCT 116 (ATCC ® CCL-247TM).

Techniques: Concentration Assay

Characterization of EVs. ( a ) Western blot for CD63, CD9, CD81, and GAPDH from two independent lots (biological replicates) of hTERT-immortalized MSC EVs. ( b ) Western blot for CD63, CD9, CD81, and GAPDH from two independent lots (biological replicates) of PC3 EVs. Multiplex analysis was performed to analyze tetraspanins (CD63, CD81, and CD9). ( c ) Average ECL values from two independent lots (biological replicates) of hTERT-immortalized MSC EVs. Each lot was assayed in duplicate. ( d ) Average ECL values from two independent lots (biological replicates) of PC3 EVs. Each lot was assayed in duplicate. (* p < 0.05; ** p < 0.01; **** p < 0.0001).

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: Characterization of EVs. ( a ) Western blot for CD63, CD9, CD81, and GAPDH from two independent lots (biological replicates) of hTERT-immortalized MSC EVs. ( b ) Western blot for CD63, CD9, CD81, and GAPDH from two independent lots (biological replicates) of PC3 EVs. Multiplex analysis was performed to analyze tetraspanins (CD63, CD81, and CD9). ( c ) Average ECL values from two independent lots (biological replicates) of hTERT-immortalized MSC EVs. Each lot was assayed in duplicate. ( d ) Average ECL values from two independent lots (biological replicates) of PC3 EVs. Each lot was assayed in duplicate. (* p < 0.05; ** p < 0.01; **** p < 0.0001).

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXMTM), PC3 (ATCC ® CRL-1435-EXMTM), and HCT 116 (ATCC ® CCL-247TM).

Techniques: Western Blot, Multiplex Assay

EV surface marker profiling and cell migration Assay. Multiplex analysis was performed to analyze various EV-associated surface marker proteins. Assays were run in duplicate. The average ECL values of each surface marker were analyzed and compared between hTERT-immortalized MSC and PC3 EVs. ( a ) THY-1, Thrombomodulin, and Neprilysin were significantly enriched in hTERT-immortalized MSC EVs. ( b ) MCAM, EPCAM, ICAM-1, EGFR, and ALCAM were significantly enriched in PC3 EVs. **** p < 0.0001; *** p < 0.001; ** p < 0.01. ( c ) A cell migration assay was performed using RPE cells. After the creation of an artificial gap, cells were immediately treated with EVs at an approximate ratio of 1:10,000 (recipient cell:EV). Representative images display gap closure over 48 h in response EVs. Scale bar = 1000 µm. ( d ) GraphPad Prism was used to analyze raw images to quantify percent gap coverage. **** p < 0.0001 relative to untreated.

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: EV surface marker profiling and cell migration Assay. Multiplex analysis was performed to analyze various EV-associated surface marker proteins. Assays were run in duplicate. The average ECL values of each surface marker were analyzed and compared between hTERT-immortalized MSC and PC3 EVs. ( a ) THY-1, Thrombomodulin, and Neprilysin were significantly enriched in hTERT-immortalized MSC EVs. ( b ) MCAM, EPCAM, ICAM-1, EGFR, and ALCAM were significantly enriched in PC3 EVs. **** p < 0.0001; *** p < 0.001; ** p < 0.01. ( c ) A cell migration assay was performed using RPE cells. After the creation of an artificial gap, cells were immediately treated with EVs at an approximate ratio of 1:10,000 (recipient cell:EV). Representative images display gap closure over 48 h in response EVs. Scale bar = 1000 µm. ( d ) GraphPad Prism was used to analyze raw images to quantify percent gap coverage. **** p < 0.0001 relative to untreated.

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXMTM), PC3 (ATCC ® CRL-1435-EXMTM), and HCT 116 (ATCC ® CCL-247TM).

Techniques: Marker, Cell Migration Assay, Multiplex Assay

Profiling of EV-associated cargo. ( a ) The results from MS show the number of unique and shared proteins between hTERT-immortalized MSC EVs and PC3 EVs. ( b ) The STRING database was used to calculate the PPIs of peptides extracted from each EV preparation. Proteins are represented by nodes that are connected by lines representative of their confidence level. Colors represent specific functions that are associated with proteins. ( c ) Total RNA was isolated from hTERT-immortalized MSCs, and RT-qPCR was performed to target candidate mRNAs. Average Cq values are shown. n = 3. ( d ) Total RNA was isolated from hTERT-immortalized MSC EVs, and RT-qPCR was performed to target candidate mRNAs. Average Cq values are shown. n = 3. ( e ) EVs were assayed for the presence of inflammatory cytokines using the S-PLEX ® proinflammatory Panel 1 Kit (Meso Scale Diagnostics) following the manufacturer’s instructions. Assays were run in duplicate. Analyte concentrations were calculated using a 4-PL fit of a standard curve of the calibrators. The data shown represent samples assayed without lysis. *** p < 0.001; * p < 0.05.

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: Profiling of EV-associated cargo. ( a ) The results from MS show the number of unique and shared proteins between hTERT-immortalized MSC EVs and PC3 EVs. ( b ) The STRING database was used to calculate the PPIs of peptides extracted from each EV preparation. Proteins are represented by nodes that are connected by lines representative of their confidence level. Colors represent specific functions that are associated with proteins. ( c ) Total RNA was isolated from hTERT-immortalized MSCs, and RT-qPCR was performed to target candidate mRNAs. Average Cq values are shown. n = 3. ( d ) Total RNA was isolated from hTERT-immortalized MSC EVs, and RT-qPCR was performed to target candidate mRNAs. Average Cq values are shown. n = 3. ( e ) EVs were assayed for the presence of inflammatory cytokines using the S-PLEX ® proinflammatory Panel 1 Kit (Meso Scale Diagnostics) following the manufacturer’s instructions. Assays were run in duplicate. Analyte concentrations were calculated using a 4-PL fit of a standard curve of the calibrators. The data shown represent samples assayed without lysis. *** p < 0.001; * p < 0.05.

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXMTM), PC3 (ATCC ® CRL-1435-EXMTM), and HCT 116 (ATCC ® CCL-247TM).

Techniques: Isolation, Quantitative RT-PCR, Lysis